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Analysis in progress: Agrobacterium vitis on grapevine rootstock for the Tunisian market

IpadLab analysis in progress Agrobacterium vitis

What do we analyse in the laboratory right now? Agrobacterium vitis on grapevine rootstock for the Tunisian market.


Why do we perform this analysis? Agrobacterium vitis analysis is required in order to certify grapevine plant material imported into Tunisia.


Analysis and strengths: IpadLab uses molecular methodologies and in particular a multiplex PCR assay that can discriminate virulent strains from non-virulent ones. This is a reliable and sensitive method which guarantees rapid analyses and results.


Description and Symptoms: The disease is known as grapevine crown gall. The symptoms are abnormal growths called "tumors" or “galls” visible on the vine trunk and on the collar.


Best analysis period: Winter and spring seasons.

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Analysis of other grapevine pathogens: Other diseases on grapevine rootstock can be analysed by IpadLab such as fungi of the genus Botryosphaeria and viruses like GFkV.


It might also be of interest to you: In winter months, IpadLab performs analysis for plant certification on the wood matrix of grapevine using the ELISA and molecular methods (official and recognised protocols). In particular, the scions are analysed for five viruses (GLRaV-1, GLRaV-3, GFLV, GVA and ArMV), while the grapevine rootstocks are analysed for six viruses (GLRaV-1, GLRaV-3, GFLV, ArMV, GVA and GFkV), as defined in the National regulation of the European countries.


IpadLab also performs molecular analysis (PLUS technology) on grapevine leaves for the diagnosis of phytoplasma from July to mid-October, and for the viruses listed above in spring and autumn.